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Phase VIII - Quantitative Transcript
Confirmation
Microarray technology allows for the detection of transcripts
that are differentially regulated in an experiment. Such transcripts
(genes) represent candidates for future genetic manipulation;
however, microarray technology is not yet adequately quantitative
enough, and real time PCR (“RT PCR”) is the method
of choice for such verification. ArrayXpress uses RT PCR technology
to quantitate the initial amount of the template; most specifically,
sensitively and reproducibly. The RT PCR system is based on
the detection and quantitation of a fluorescent reporter. This
signal increases in direct proportion to the amount of PCR product
in a reaction. By recording the amount of fluorescence emission
at each cycle, it is possible to monitor the PCR reaction during
the exponential phase where the first significant increase in
the amount of PCR product correlates to the initial amount of
target template, providing a much more quantitative estimation
of transcript levels.
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Superior Quality
The best raw data, experiment results and analysis
available in the industry
Complete Solution
The first and only commercial one-stop shop for
microarray services
Fully Customizable
Any size, any organism, any method, any microarray
experiment
Lower Cost
Save now (lower project cost) and save later (lower
downstream costs)
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