Scanning and image analysis are critical steps in the microarray process to assure that high quality raw data is retrieved. In general, photon energy from emitted fluorescent light is converted into electrical energy, thus producing a measurable signal that is proportional to the number of photons detected. These analog signals are then converted into digital signals representing relative transcript intensity levels. Data is then extracted using QuantArray software (GSI Lumonics). Quality control steps are incorporated to ensure overall quality for each array image.